Example Program

This simple example demonstrates using loops and variables to transfer between wells.

example.py

from pathlib import Path
from pyalab import Deck, DeckLayout, DeckPositions, Pipette, Plate, Program, SetInitialVolume, SetVolume, StandardDeckNames, Tip, Transfer

pcr_plate = Plate(name="BIO-RAD Hard-Shell 96-Well Skirted PCR Plates", display_name="PCR Plate")
program = Program(
    deck_layouts=[
        DeckLayout(
            deck=Deck(name=StandardDeckNames.THREE_POSITION.value),
            labware={DeckPositions.B_PLATE_LANDSCAPE.value: pcr_plate},
        )
    ],
    display_name="simple-transfer",
    description="Transfer in 96-well plate",
    pipette=Pipette(name="VOYAGER EIGHT 300 µl"),
    tip=Tip(name="300 µl GripTip Sterile Filter Low retention"),
)
pcr_plate_section_index = program.get_section_index_for_plate(pcr_plate)

program.add_step(
    SetInitialVolume(
        plate=pcr_plate,
        section_index=pcr_plate_section_index,
        column_index=0,
        volume=200,
    )
)
for column_index in range(1, 12):
    program.add_step(
        SetVolume(
            plate=pcr_plate,
            section_index=pcr_plate_section_index,
            column_index=column_index,
            volume=0,
        )
    )

transfer_volume = 18
for column_index in range(1, 12):
    program.add_step(
        Transfer(
            source=pcr_plate,
            source_section_index=pcr_plate_section_index,
            source_column_index=0,
            destination=pcr_plate,
            destination_section_index=pcr_plate_section_index,
            destination_column_index=column_index,
            volume=transfer_volume,
        )
    )
    transfer_volume -= 1
program.dump_xml(Path(__file__).parent / "simple-transfer.iaa")